Opening of the replication origin of Escherichia coli by DnaA protein with protein HU or IHF.
Identifieur interne : 004720 ( Main/Exploration ); précédent : 004719; suivant : 004721Opening of the replication origin of Escherichia coli by DnaA protein with protein HU or IHF.
Auteurs : D S Hwang [États-Unis] ; A. KornbergSource :
- The Journal of biological chemistry [ 0021-9258 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), ADN bactérien (isolement et purification), Cartographie de restriction, Cinétique, Données de séquences moléculaires, Escherichia coli (génétique), Escherichia coli (métabolisme), Facteurs d'intégration de l'hôte, Gènes bactériens, Liaison aux protéines, Oligodésoxyribonucléotides, Plasmides, Protéines bactériennes (métabolisme), Protéines de liaison à l'ADN (métabolisme), Réplication de l'ADN, Séquence nucléotidique.
- MESH :
- génétique : ADN bactérien, Escherichia coli.
- isolement et purification : ADN bactérien.
- métabolisme : Escherichia coli, Protéines bactériennes, Protéines de liaison à l'ADN.
- Cartographie de restriction, Cinétique, Données de séquences moléculaires, Facteurs d'intégration de l'hôte, Gènes bactériens, Liaison aux protéines, Oligodésoxyribonucléotides, Plasmides, Réplication de l'ADN, Séquence nucléotidique.
English descriptors
- KwdEn :
- Bacterial Proteins (metabolism), Base Sequence, DNA Replication, DNA, Bacterial (genetics), DNA, Bacterial (isolation & purification), DNA-Binding Proteins (metabolism), Escherichia coli (genetics), Escherichia coli (metabolism), Genes, Bacterial, Integration Host Factors, Kinetics, Molecular Sequence Data, Oligodeoxyribonucleotides, Plasmids, Protein Binding, Restriction Mapping.
- MESH :
- chemical , genetics : DNA, Bacterial.
- chemical , isolation & purification : DNA, Bacterial.
- chemical , metabolism : Bacterial Proteins, DNA-Binding Proteins.
- genetics : Escherichia coli.
- metabolism : Escherichia coli.
- Base Sequence, DNA Replication, Genes, Bacterial, Integration Host Factors, Kinetics, Molecular Sequence Data, Oligodeoxyribonucleotides, Plasmids, Protein Binding, Restriction Mapping.
Abstract
Opening of the three tandem repeats of a 13-mer in the replication origin (oriC) of Escherichia coli is a prime event in the replication in vitro of minichromosomes (Bramhill, D., and Kornberg, A. (1988) Cell 54, 915-918). DnaA, the initiator protein, requires protein HU or IHF, along with a millimolar level of ATP and negative superhelical density in the plasmid to open this region. The extent of opening, as judged by cleavage by a single-strand-specific endonuclease (i.e. P1 nuclease), correlated closely with replication of the oriC plasmid. In an initial complex, preceding opening of the 13-mers, the footprint of DnaA protein bound by ATP covered its four 9-mer recognition sequences. The footprint of the nucleotide-free form of the protein, by contrast, was more extensive and thus, less specific.
PubMed: 1429655
Affiliations:
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Le document en format XML
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Kornberg</name></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="ISSN">0021-9258</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (metabolism)</term><term>Base Sequence</term><term>DNA Replication</term><term>DNA, Bacterial (genetics)</term><term>DNA, Bacterial (isolation & purification)</term><term>DNA-Binding Proteins (metabolism)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Genes, Bacterial</term><term>Integration Host Factors</term><term>Kinetics</term><term>Molecular Sequence Data</term><term>Oligodeoxyribonucleotides</term><term>Plasmids</term><term>Protein Binding</term><term>Restriction Mapping</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>ADN bactérien (isolement et purification)</term><term>Cartographie de restriction</term><term>Cinétique</term><term>Données de séquences moléculaires</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Facteurs d'intégration de l'hôte</term><term>Gènes bactériens</term><term>Liaison aux protéines</term><term>Oligodésoxyribonucléotides</term><term>Plasmides</term><term>Protéines bactériennes (métabolisme)</term><term>Protéines de liaison à l'ADN (métabolisme)</term><term>Réplication de l'ADN</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term><term>DNA-Binding Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN bactérien</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term><term>Protéines bactériennes</term><term>Protéines de liaison à l'ADN</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>DNA Replication</term><term>Genes, Bacterial</term><term>Integration Host Factors</term><term>Kinetics</term><term>Molecular Sequence Data</term><term>Oligodeoxyribonucleotides</term><term>Plasmids</term><term>Protein Binding</term><term>Restriction Mapping</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie de restriction</term><term>Cinétique</term><term>Données de séquences moléculaires</term><term>Facteurs d'intégration de l'hôte</term><term>Gènes bactériens</term><term>Liaison aux protéines</term><term>Oligodésoxyribonucléotides</term><term>Plasmides</term><term>Réplication de l'ADN</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Opening of the three tandem repeats of a 13-mer in the replication origin (oriC) of Escherichia coli is a prime event in the replication in vitro of minichromosomes (Bramhill, D., and Kornberg, A. (1988) Cell 54, 915-918). DnaA, the initiator protein, requires protein HU or IHF, along with a millimolar level of ATP and negative superhelical density in the plasmid to open this region. The extent of opening, as judged by cleavage by a single-strand-specific endonuclease (i.e. P1 nuclease), correlated closely with replication of the oriC plasmid. In an initial complex, preceding opening of the 13-mers, the footprint of DnaA protein bound by ATP covered its four 9-mer recognition sequences. The footprint of the nucleotide-free form of the protein, by contrast, was more extensive and thus, less specific.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><noCountry><name sortKey="Kornberg, A" sort="Kornberg, A" uniqKey="Kornberg A" first="A" last="Kornberg">A. Kornberg</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Hwang, D S" sort="Hwang, D S" uniqKey="Hwang D" first="D S" last="Hwang">D S Hwang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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